Human RYBP Recombinant Protein (RPPB4543)
- SKU:
- RPPB4543
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q8N488
Description
| Product Name: | Human RYBP Recombinant Protein |
| Product Code: | RPPB4543 |
| Size: | 10µg |
| Species: | Human |
| Target: | RYBP |
| Synonyms: | RING1 and YY1 binding protein, Death effector domain-associated factor, Apoptin-associating protein 1, YY1 and E4TF1-associated factor 1, ring1 interactor RYBP, DED-associated factor, APAP-1, DEDAF, YEAF1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The RYBP solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMTMGDK KSPTRPKRQA KPAADEGFWD CSVCTFRNSA EAFKCSICDV RKGTSTRKPR INSQLVAQQV AQQYATPPPP KKEKKEKVEK QDKEKPEKDK EISPSVTKKN TNKKTKPKSD ILKDPPSEAN SIQSANATTK TSETNHTSRP RLKNVDRSTA QQLAVTVGNV TVIITDFKEK TRSSSTSSST VTSSAGSEQQ NQSSSGSEST DKGSSRSSTP KGDMSAVNDE SF |
RING1- and YY1-binding protein (RYBP) belongs to the polycomb group (PcG). RYBP interacts with MDM2 and decreases MDM2-mediated p53 ubiquitination, which leads to stabilization of p53 and an increase in p53 activity. RYBP causes cell-cycle arrest and is involved in the p53 response to DNA damage. RYBP interacts with RING1, YY1, Caspase 10, E2F3, E2F2, Mdm2, Abl gene and CBX2. RYBP inhibits ubiquitination and subsequent degradation of TP53, and thus has a role in regulating transcription of TP53 target genes. RYBP may also be involved in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. RYBP may bind to DNA, and promote apoptosis.
RYBP Human Recombinant produced in E. coli is a single polypeptide chain containing 252 amino acids (1-228) and having a molecular mass of 27.4 kDa.RYBP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RYBP: Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. Promotes apoptosis. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 3p13 Cellular Component: nucleoplasm; PcG protein complex Molecular Function:protein binding Biological Process: multicellular organismal development |
| UniProt Code: | Q8N488 |
| NCBI GenInfo Identifier: | 78102506 |
| NCBI Gene ID: | 23429 |
| NCBI Accession: | Q8N488.2 |
| UniProt Secondary Accession: | Q8N488,Q9P2W5, Q9UMW4, |
| UniProt Related Accession: | Q8N488 |
| Molecular Weight: | 24,822 Da |
| NCBI Full Name: | RING1 and YY1-binding protein |
| NCBI Synonym Full Names: | RING1 and YY1 binding protein |
| NCBI Official Symbol: | RYBP�� |
| NCBI Official Synonym Symbols: | AAP1; DEDAF; YEAF1; APAP-1�� |
| NCBI Protein Information: | RING1 and YY1-binding protein |
| UniProt Protein Name: | RING1 and YY1-binding protein |
| UniProt Synonym Protein Names: | Apoptin-associating protein 1; APAP-1; Death effector domain-associated factor; DED-associated factor; YY1 and E4TF1-associated factor 1 |
| Protein Family: | RING1 and YY1-binding protein |
| UniProt Gene Name: | RYBP�� |
| UniProt Entry Name: | RYBP_HUMAN |
