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Human Rubella Virus (RV) IgG ELISA Kit (AEES00744)

SKU:
AEES00744
Product Type:
ELISA Kit
Size:
96 Assays
Reactivity:
Human
Target:
RV IgG
$599
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name: Human Rubella Virus (RV) IgG ELISA Kit
SKU: AEES00744
Target: RV IgG
Reactivity: Human
Size: 96T
Assay type: Indirect ELISA
Detection Wavelength: 450/630nm
Sample type: Serum
Storage: 12 months from the production date at 2-8℃
Kit component:
Component Quantity (96 Assays)
ELISA Microtiter plate 96 wells
Positive Control 1 mL
Negative Control 1 mL
HRP Conjugate 12 mL
Sample Diluent 12 mL
20×Concentrated Wash Buffer 50 mL
Substrate Reagent A 6 mL
Substrate Reagent B 6 mL
Stop Solution 6 mL
Plate Sealer 3 pieces
Product Manual 1 copy

Follow the step-by-step procedure below to carry out your ELISA assay efficiently.

Step Protocol
Number Number the samples and controls in order across multiple wells and keep a record of control wells and sample wells. Set 1 well for blank control, 3 wells for negative control, and 1 well for positive control. Test samples in duplicate. (Blank well is not necessary for dual-wavelength detection.)
Add Sample

a) Add 100 μL of negative/positive control respectively to 3 negative control wells and 1 positive control well. Keep the blank control well empty.

b) Dilute the serum being tested with Sample Diluent at a 1:10 ratio (add 100 μL of Sample Diluent to the reaction well, then add 10 μL of serum sample), and mix thoroughly.
Incubate Gently tap the plate to mix thoroughly. Cover the ELISA plate with a sealer and incubate for 30 minutes at 37℃ in the dark.
Wash Remove the plate sealer and aspirate the liquid from each well. Repeat the washing procedure 5 times with Wash Buffer, allowing the buffer to immerse for 30-60 seconds each time. Invert the plate and tap it against thick clean absorbent paper. (If bubbles form in the wells, use clean tips to prick them.)
HRP Conjugate Add 100 μL of HRP Conjugate to each well except the blank control well.
Incubate Cover the ELISA plate with a sealer and incubate for 30 minutes at 37℃ in the dark.
Wash Repeat the washing procedure as described in step 4.
Add Substrate Add 50 μL of Substrate Reagent A and 50 μL of Substrate Reagent B to each well. Cover the plate with a sealer, mix thoroughly, and incubate for 10 minutes at 37℃ in the dark.
Stop Reaction Add 50 μL of Stop Solution to each well and gently tap the plate to mix thoroughly.
OD Measurement Set the Micro-plate Reader to a wavelength of 450 nm (dual-wavelength setting of 450 nm/630 nm is recommended) to measure the A value of each well. Blank wells are not essential when using dual-wavelength detection. Note: Read the results within 30 minutes.

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