Human RPRD1B Recombinant Protein (RPPB4505)
- SKU:
- RPPB4505
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9NQG5
Description
| Product Name: | Human RPRD1B Recombinant Protein |
| Product Code: | RPPB4505 |
| Size: | 20µg |
| Species: | Human |
| Target: | RPRD1B |
| Synonyms: | Regulation of nuclear pre-mRNA domain-containing protein1B, C20orf77, CREPT, dJ1057B20.2, NET60. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | RPRD1B protein solution (1mg/ml) in Phosphate buffered saline (pH7.4), 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHHSSGLVPRGSH MGSMSSFSESALEKKLSELS NSQQSVQTLS LWLIHHRKHA GPIVSVWHRE LRKAKSNRKL TFLYLANDVI QNSKRKGPEFTREFESVLVD AFSHVAREAD EGCKKPLERL LNIWQERSVY GGEFIQQLKL SMEDSKSPPP KATEEKKSLKRTFQQIQEEE DDDYPGSYSP QDPSAGPLLT EELIKALQDL ENAASGDATV RQKIASLPQE VQDVSLLEKITDKEAAERLS KTVDEACLLL AEYNGRLAAE LEDRRQLARM LVEYTQNQKD VLSEKEKKLE EYKQKLARVTQVRKELKSHI QSLPDLSLLP NVTGGLAPLP SAGDLFSTD |
Regulation of nuclear pre-mRNA domain-containing protein 1B (RPRD1B) interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. RPRD1B stimulates binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. RPRD1B inhibits RNA polymerase II from reading through the 3' end termination site and may allow it to be drafted back to the promoter through promotion of the formation of a chromatin loop. In addition, RPRD1B enhances the transcription of several other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. RPRD1B also promotes cell proliferation.
RPRD1B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 349 amino acids (1-326 a.a) and having a molecular mass of 39.3kDa.RPRD1B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RPRD1B: Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. Belongs to the UPF0400 (RTT103) family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 20q11.23 Cellular Component: centrosome; cytosol; DNA-directed RNA polymerase II, holoenzyme; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter |
| UniProt Code: | Q9NQG5 |
| NCBI GenInfo Identifier: | 23813907 |
| NCBI Gene ID: | 58490 |
| NCBI Accession: | Q9NQG5.1 |
| UniProt Secondary Accession: | Q9NQG5,Q1WDE7, Q6PKF4, |
| UniProt Related Accession: | Q9NQG5 |
| Molecular Weight: | 37kDa |
| NCBI Full Name: | Regulation of nuclear pre-mRNA domain-containing protein 1B |
| NCBI Synonym Full Names: | regulation of nuclear pre-mRNA domain containing 1B |
| NCBI Official Symbol: | RPRD1B�� |
| NCBI Official Synonym Symbols: | CREPT; NET60; C20orf77; dJ1057B20.2�� |
| NCBI Protein Information: | regulation of nuclear pre-mRNA domain-containing protein 1B |
| UniProt Protein Name: | Regulation of nuclear pre-mRNA domain-containing protein 1B |
| UniProt Synonym Protein Names: | Cell cycle-related and expression-elevated protein in tumor |
| Protein Family: | Regulation of nuclear pre-mRNA domain-containing protein |
| UniProt Gene Name: | RPRD1B�� |
