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Human RPN2 Recombinant Protein (RPPB2258)

SKU:
RPPB2258
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P04844
Research Area:
Enzymes
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Description

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Product Name:Human RPN2 Recombinant Protein
Product Code:RPPB2258
Size:20µg
Species:Human
Target:RPN2
Synonyms:Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit, RIBIIR, Ribophorin II, RPN-II, Ribophorin-2, RPN2, SWP1, RPNII.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The RPN2 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MLTPTHYLTK HDVERLKASL DRPFTNLESA FYSIVGLSSL GAQVPDAKKA CTYIRSNLDP SNVDSLFYAA QASQALSGCE ISISNETKDL LLAAVSEDSS VTQIYHAVAA LSGFGLPLAS QEALSALTAR LSKEETVLAT VQALQTASHL SQQADLRSIVEEIEDLVARL DELGGVYLQF EEGLETTALF VAATYKLMDH VGTEPSIKED QVIQLMNAIF SKKNFESLSE AFSVASAAAV LSHNRYHVPV VVVPEGSASD THEQAILRLQ VTNVLSQPLT QATVKLEHAK SVASRATVLQ KTSFTPVGDV FELNFMNVKF SSGYYDFLVE VEGDNRYIANTVELRVKIST EVGITNVDLS TVDKDQSIAP KTTRVTYPAK AKGTFIADSH QNFALFFQLV DVNTGAELTP HQTFVRLHNQ KTGQEVVFVA EPDNKNVYKF ELDTSERKIE FDSASGTYTL YLIIGDATLK NPILWNVADV VIKFPEEEAP STVLSQNLFT PKQEIQHLFR EPEKRPPTV

Ribophorin 2 (RPN2) is a dolichyl-diphosphooligosaccharide protein glycosyltransferase subunit 2. The Ribophorin 2 protein is part of an N-oligosaccharyl transferase complex which links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. RPN2 is analogous in sequence to the yeast oligosaccharyl transferase subunit SWP1.

RPN2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 539 amino acids (23-540) and having a molecular mass of 59.2kDa.RPN2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:RPN2: Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Belongs to the SWP1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.4.99.18; Transferase; Membrane protein, multi-pass; Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis

Chromosomal Location of Human Ortholog: 20q12-q13.1

Cellular Component: endoplasmic reticulum membrane; rough endoplasmic reticulum; membrane; endoplasmic reticulum; oligosaccharyl transferase complex; integral to membrane; nucleus

Molecular Function:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; ribosome binding

Biological Process: response to drug; SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; protein modification process; gene expression; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; aging

NCBI Summary:This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Code:P04844
NCBI GenInfo Identifier:9297108
NCBI Gene ID:6185
NCBI Accession:P04844.3
UniProt Secondary Accession:P04844,Q5JYR6, Q6IBA5, Q96E21, Q9BUQ3, Q9UBE1,
UniProt Related Accession:P04844
Molecular Weight:67,723 Da
NCBI Full Name:Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
NCBI Synonym Full Names:ribophorin II
NCBI Official Symbol:RPN2��
NCBI Official Synonym Symbols:SWP1; RPNII; RIBIIR; RPN-II��
NCBI Protein Information:dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; ribophorin-2; oligosaccharyltransferase complex subunit (non-catalytic); dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
UniProt Protein Name:Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
UniProt Synonym Protein Names:Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit; RIBIIR; Ribophorin II; RPN-II; Ribophorin-2
Protein Family:Dolichyl-diphosphooligosaccharide--protein glycosyltransferase
UniProt Gene Name:RPN2��
UniProt Entry Name:RPN2_HUMAN

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