Human RPA2 Recombinant Protein (RPPB4486)
- SKU:
- RPPB4486
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P15927
Description
| Product Name: | Human RPA2 Recombinant Protein |
| Product Code: | RPPB4486 |
| Size: | 10µg |
| Species: | Human |
| Target: | RPA2 |
| Synonyms: | Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34, RPA2, REPA2, RPA32, RPA34. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The RPA2 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMWNSGFE SYGSSSYGGA GGYTQSPGGF GSPAPSQAEK KSRARAQHIV PCTISQLLSA TLVDEVFRIG NVEISQVTIV GIIRHAEKAP TNIVYKIDDM TAAPMDVRQW VDTDDTSSEN TVVPPETYVK VAGHLRSFQN KKSLVAFKIM PLEDMNEFTT HILEVINAHM VLSKANSQPS AGRAPISNPG MSEAGNFGGN SFMPANGLTV AQNQVLNLIK ACPRPEGLNF QDLKNQLKHM SVSSIKQAVD FLSNEGHIYS TVDDDHFKST DAE |
Replication Protein A2 (RPA2) is a single stranded DNA binding protein. Human RPA2 is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. The RPA2 protein complex is highly conserved in eukaryotes and is crucial in DNA replication, homologous recombination and nucleotide excision repair. RPA2 C-terminus specifically interacts with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. Additionally, RPA2 binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA2 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3.
RPA2 Human Recombinant fused with a 23 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 293 amino acids (1-270 a.a.) and having a molecular mass of 31.7kDa. The RPA2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RFA2: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage. Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA replication Chromosomal Location of Human Ortholog: 1p35 Cellular Component: chromatin; chromosome, telomeric region; DNA replication factor A complex; nuclear chromosome, telomeric region; nucleoplasm; nucleus; PML body Molecular Function:damaged DNA binding; enzyme binding; protein binding; protein N-terminus binding; protein phosphatase binding; single-stranded DNA binding; ubiquitin protein ligase binding Biological Process: base-excision repair; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA repair; DNA replication; DNA strand elongation during DNA replication; double-strand break repair; double-strand break repair via homologous recombination; error-prone postreplication DNA repair; G1 DNA damage checkpoint; G1/S transition of mitotic cell cycle; mismatch repair; mitotic cell cycle; nucleotide-excision repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; telomere maintenance; telomere maintenance via recombination; telomere maintenance via semi-conservative replication; transcription-coupled nucleotide-excision repair |
| UniProt Code: | P15927 |
| NCBI GenInfo Identifier: | 132474 |
| NCBI Gene ID: | 6118 |
| NCBI Accession: | P15927.1 |
| UniProt Secondary Accession: | P15927,Q52II0, Q5TEI9, Q5TEJ5, |
| UniProt Related Accession: | P15927 |
| Molecular Weight: | 38,810 Da |
| NCBI Full Name: | Replication protein A 32 kDa subunit |
| NCBI Synonym Full Names: | replication protein A2 |
| NCBI Official Symbol: | RPA2�� |
| NCBI Official Synonym Symbols: | REPA2; RPA32; RP-A p32; RP-A p34�� |
| NCBI Protein Information: | replication protein A 32 kDa subunit |
| UniProt Protein Name: | Replication protein A 32 kDa subunit |
| UniProt Synonym Protein Names: | Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34 |
| Protein Family: | Replication protein |
| UniProt Gene Name: | RPA2�� |
| UniProt Entry Name: | RFA2_HUMAN |
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