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Human Replication Protein A1 (RPA1) ELISA Kit

SKU:
HUDL02562
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27694
Sensitivity:
0.106ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HSSB,REPA1,RF-A,RP-A,RPA70,Replication protein A 70 kDa DNA-binding subunit,Single-stranded DNA-binding protein
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Replication Protein A1 (RPA1) ELISA Kit

The Human Replication Protein A1 (RPA1) ELISA Kit is specifically designed for the accurate measurement of RPA1 levels in human samples, including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an ideal tool for various research applications.RPA1 is a key protein involved in DNA replication and repair processes. Its dysregulation has been implicated in various diseases, including cancer and genetic disorders.

By detecting and quantifying RPA1 levels, researchers can gain valuable insights into these disease mechanisms and potentially develop targeted therapies.Overall, the Human RPA1 ELISA Kit offers a valuable tool for researchers studying DNA replication, repair, and associated diseases. Its accurate and precise measurements make it a reliable asset for advancing scientific knowledge and potential clinical applications.

Product Name:Human Replication Protein A1 (RPA1) ELISA Kit
Product Code:HUDL02562
Size:96 Assays
Target:RPA1
Synonyms:HSSB, REPA1, RF-A, RP-A, RPA70, Replication protein A 70 kDa DNA-binding subunit, Single-stranded DNA-binding protein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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