Human RNF4 Recombinant Protein (RPPB4478)
- SKU:
- RPPB4478
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P78317
Description
| Product Name: | Human RNF4 Recombinant Protein |
| Product Code: | RPPB4478 |
| Size: | 20µg |
| Species: | Human |
| Target: | RNF4 |
| Synonyms: | E3 ubiquitin-protein ligase RNF4, RING finger protein 4, Small nuclear ring finger protein, Protein SNURF, RNF4, SNURF, RES4-26, SLX5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | RNF4 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.5), 0.2M NaCl, 30% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSTRKRR GGAINSRQAQ KRTREATSTP EISLEAEPIE LVETAGDEIV DLTCESLEPV VVDLTHNDSV VIVDERRRPR RNARRLPQDH ADSCVVSSDD EELSRDRDVY VTTHTPRNAR DEGATGLRPS GTVSCPICMD GYSEIVQNGR LIVSTECGHV FCSQCLRDSL KNANTCPTCR KKINHKRYHP IYI |
E3 ubiquitin ligase RNF4 (RNF4) contains a RING finger motif and functions as a transcription regulator. RNF4 interacts with, and inhibits the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ also interacts with the RNF4 protein thus reducing the enhancement of androgen receptor-dependent transcription mediated by RNF4. RNF4 is broadly expressed at low levels in many tissues and highly expressed in the testis.
RNF4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 213 amino acids (1-190 a.a) and having a molecular mass of 23.7kDa.RNF4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RNF4: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; Ligase; Nuclear receptor co-regulator; Transcription factor; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 4p16.3 Cellular Component: cytoplasm; nucleoplasm; nucleus; PML body Molecular Function:DNA binding; identical protein binding; nucleosome binding; protein binding; SUMO polymer binding; transcription factor activity; ubiquitin-protein ligase activity Biological Process: positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; protein ubiquitination; response to arsenic |
| NCBI Summary: | The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.[provided by RefSeq, Jul 2010] |
| UniProt Code: | P78317 |
| NCBI GenInfo Identifier: | 18202358 |
| NCBI Gene ID: | 6047 |
| NCBI Accession: | P78317.1 |
| UniProt Secondary Accession: | P78317,Q49AR8, B2R6D6, D6RF58, |
| UniProt Related Accession: | P78317 |
| Molecular Weight: | 21kDa |
| NCBI Full Name: | E3 ubiquitin-protein ligase RNF4 |
| NCBI Synonym Full Names: | ring finger protein 4 |
| NCBI Official Symbol: | RNF4�� |
| NCBI Official Synonym Symbols: | SLX5; SNURF; RES4-26�� |
| NCBI Protein Information: | E3 ubiquitin-protein ligase RNF4 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase RNF4 |
| UniProt Synonym Protein Names: | RING finger protein 4; RING-type E3 ubiquitin transferase RNF4Curated; Small nuclear ring finger protein; Protein SNURF |
| Protein Family: | E3 ubiquitin-protein ligase |
| UniProt Gene Name: | RNF4�� |
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