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Human RNASE3 Recombinant Protein (RPPB2246)

SKU:
RPPB2246
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P12724
Research Area:
Enzymes
€309
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Description

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Product Name:Human RNASE3 Recombinant Protein
Product Code:RPPB2246
Size:20µg
Species:Human
Target:RNASE3
Synonyms:ECP, RNS3, Ribonuclease 3, Eosinophil cationic protein, RNASE3, RNASE3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The RNASE3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRP PQFTRAQWFA IQHISLNPPR CTIAMRAINN YRWRCKNQNT FLRTTFANVV NVCGNQSIRC PHNRTLNNCH RSRFRVPLLH CDLINPGAQN ISNCRYADRP GRRFYVVACD NRDPRDSPRY PVVPVHLDTT I

Ribonuclease 3 (RNASE3) is cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. RNASE3 possesses a broad diversity of biological activities. RNASE3 protein has shown antibacterial activity such as cytoplasmic membrane depolarization of preferentially Gram-negative and Gram-positive strains and promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

RNASE3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 171 amino acids (28-160) and having a molecular mass of 19.9kDa. RNASE3 is fused to a 38 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:RNASE3: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Belongs to the pancreatic ribonuclease family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; EC 3.1.27.-; Ribonuclease

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space; extracellular region

Molecular Function:nucleic acid binding; endonuclease activity; ribonuclease activity

Biological Process: defense response to Gram-positive bacterium; innate immune response in mucosa; RNA catabolic process; antibacterial humoral response

NCBI Summary:The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]
UniProt Code:P12724
NCBI GenInfo Identifier:147744558
NCBI Gene ID:6037
NCBI Accession:P12724.2
UniProt Secondary Accession:P12724,Q4VBC1, Q8WTP7, Q8WZ62, Q9GZN9,
UniProt Related Accession:P12724
Molecular Weight:18kDa
NCBI Full Name:Eosinophil cationic protein
NCBI Synonym Full Names:ribonuclease, RNase A family, 3
NCBI Official Symbol:RNASE3��
NCBI Official Synonym Symbols:ECP; RNS3��
NCBI Protein Information:eosinophil cationic protein; RNase 3; ribonuclease 3; cytotoxic ribonuclease
UniProt Protein Name:Eosinophil cationic protein
UniProt Synonym Protein Names:Ribonuclease 3; RNase 3
Protein Family:Eosinophil cationic protein
UniProt Gene Name:RNASE3��
UniProt Entry Name:ECP_HUMAN

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