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Human Ribosome-binding protein 1 / RRBP1 ELISA Kit

SKU:
HUFI00954
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9P2E9
Sensitivity:
46.875pg/ml
Range:
78.125-5000pg/ml
ELISA Type:
Sandwich
Synonyms:
RRBP1, Ribosome-binding protein 1, Ribosome receptor protein, 180 kDa ribosome receptor homolog, RRp
Reactivity:
Human
Research Area:
Cell Biology
€599
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Description

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Human Ribosome-binding protein 1 / RRBP1 ELISA Kit

RRBP1 has been linked to endoplasmic reticulum (ER) cell growth, secretion pathways, and secretory cell differentiation. RRBP1 may regulates ER-microtubule interactions as well. The ribosome-binding affinity and secretory function vary as a result of splicing variations. Epithelioid inflammatory myofibroblastic sarcoma and Flinders island spotted fever are two examples of RRBP1-related diseases. The Assay Genie Ribosome-binding protein 1/RRBP1 ELISA is a highly sensitive assay for the quantitative measurement of Ribosome-binding protein 1/RRBP1 in serum, blood, plasma, cell culture supernatant and tissue samples.

Product Name:Human Ribosome-binding protein 1 / RRBP1 ELISA Kit
Product Code:HUFI00954
Size:96 Assays
Alias:RRBP1, Ribosome-binding protein 1, Ribosome receptor protein, 180 kDa ribosome receptor homolog, RRp
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human RRBP1 concentrations in serum plasma and other biological fluids.
Sensitivity:46.875pg/ml
Range:78.125-5000pg/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human RRBP1 and the recovery rates were calculated by comparing the measured value to the expected amount of Human RRBP1 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)92-10598
EDTA plasma(n=5)86-10390
UFH plasma(n=5)89-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human RRBP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)85-96%90-105%88-102%
EDTA plasma(n=5)84-95%85-98%87-94%
UFH plasma(n=5)82-99%87-98%87-98%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotQ9P2E9
UniProt Protein Function:RRBP1: a single-pass type III membrane protein of the rough endoplasmic reticulum membrane. Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. Associated with the unfolded protein response (UPR) and has anti-apoptotic activity. Knockdown of RRBP1 induces ER stress, and is associated with a significant reduction in the expression of glucose-regulated protein 78 (GRP78). GRP78 regulates the UPR, is widely upregulated in cancer, and plays an important role in chemotherapy resistance in some cancers. Upregulated in many cancers: overexpressed in a high proportion of breast carcinoma cases tested, and is highly expressed in lung cancer tissue compared with adjacent normal tissues. Because RRBP1 appears to play a role in tumor cell survival, it may be a candidate for new targeted therapeutics. Three human isoforms are produced by alternative splicing.
UniProt Protein Details:Protein type:Membrane protein, integral; Translation; Ribosomal

Chromosomal Location of Human Ortholog: 20p12

Cellular Component: endoplasmic reticulum; integral to endoplasmic reticulum membrane; membrane; ribosome

Molecular Function:receptor activity

Biological Process: osteoblast differentiation; translation

NCBI Summary:This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12. [provided by RefSeq, Apr 2012]
UniProt Code:Q9P2E9
NCBI GenInfo Identifier:23822112
NCBI Gene ID:6238
NCBI Accession:Q9P2E9.4
UniProt Secondary Accession:Q9P2E9,O75300, O75301, Q5W165, Q96SB2, Q9BWP1, Q9H476 A2A2S6, A6NCN6,
UniProt Related Accession:Q9P2E9
Molecular Weight:108,632 Da
NCBI Full Name:Ribosome-binding protein 1
NCBI Synonym Full Names:ribosome binding protein 1
NCBI Official Symbol:RRBP1
NCBI Official Synonym Symbols:RRp; hES; ES130; ES/130
NCBI Protein Information:ribosome-binding protein 1
UniProt Protein Name:Ribosome-binding protein 1
UniProt Synonym Protein Names:180 kDa ribosome receptor homolog; RRp; ES/130-related protein; Ribosome receptor protein
Protein Family:Ribosome-binding protein
UniProt Gene Name:RRBP1
UniProt Entry Name:RRBP1_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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