The Human Ribonuclease H (RNase H) ELISA Kit is a powerful tool for detecting and quantifying levels of RNase H in human biological samples. RNase H is an enzyme that plays a key role in the process of nucleic acid metabolism, making it an important target for research in various fields such as molecular biology, genetics, and drug development.This ELISA kit offers high sensitivity and specificity, ensuring accurate and reliable results for your experiments. It is suitable for use with human serum, plasma, and cell culture supernatants, providing versatility for different sample types.RNase H is involved in various biological processes, including DNA replication, DNA repair, and RNA degradation.
Dysregulation of RNase H activity has been implicated in diseases such as cancer, HIV infection, and neurodegenerative disorders, making it a valuable biomarker for disease research and therapeutic development.With the Human RNase H ELISA Kit, you can easily measure and analyze RNase H levels in your samples, advancing your understanding of nucleic acid metabolism and its implications in human health and disease. Trust in the accuracy and precision of this kit to support your research goals effectively.
Product Name:
Human Ribonuclease H (RNASEH) ELISA Kit
Product Code:
HUDL02549
Size:
96 Assays
Target:
RNASEH
Synonyms:
Rnase-H, RNASEH1, RNH1, Ribonuclease H type II
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
Nguyen et al.
Chemical evolution of an autonomous DNAzyme with allele-specific gene silencing activity
