Human Rho GDP Dissociation Inhibitor Alpha (ARHGDIa) ELISA Kit
The Human Rho GDP Dissociation Inhibitor alpha (ARHGDIA) ELISA Kit is a highly sensitive and specific tool for the accurate detection of ARHGDIA levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.ARHGDIA, also known as RhoGDI alpha, plays a crucial role in regulating Rho family GTPases, which are key signaling molecules involved in various cellular processes such as cell migration, adhesion, and proliferation.
Dysregulation of ARHGDIA has been implicated in cancer metastasis, cardiovascular diseases, and neurological disorders, highlighting its importance as a potential biomarker and therapeutic target.By accurately measuring ARHGDIA levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic strategies. The Human ARHGDIA ELISA Kit from Assay Genie offers a reliable and efficient solution for studying the role of ARHGDIA in health and disease.
Product Name:
Human Rho GDP Dissociation Inhibitor Alpha (ARHGDIa) ELISA Kit
Product Code:
HUDL00239
Size:
96 Assays
Target:
ARHGDIa
Synonyms:
GDIA1, RHOGDI, RHOGDI-1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
