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Human Rh Blood Group, D Antigen (RHD) ELISA Kit

SKU:
HUDL02533
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q02161
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD240D,Rh30a,Rh4,RhPI,RhII,DIIIc,Rhesus D antigen,Rh polypeptide 2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Human Rh Blood Group, D Antigen (RHD) ELISA Kit

The Human RH Blood Group D Antigen (RHD) ELISA Kit is specifically designed for the precise detection of the RHD antigen in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.The RHD antigen is a critical component of the RH blood group system, with implications for blood transfusion compatibility and hemolytic disease of the newborn.

Accurate detection of the RHD antigen is essential for blood typing and transfusion medicine, as well as for understanding the genetic basis of RH blood group variants.With its high-quality components and user-friendly protocol, the Human RH Blood Group D Antigen (RHD) ELISA Kit is an invaluable tool for researchers and clinicians alike, providing valuable insights into blood group antigen expression and compatibility.

Product Name:Human Rh Blood Group, D Antigen (RHD) ELISA Kit
Product Code:HUDL02533
Size:96 Assays
Target:RHD
Synonyms:CD240D, Rh30a, Rh4, RhPI, RhII, DIIIc, Rhesus D antigen, Rh polypeptide 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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