The Human Reticulocalbin-2 (RCN2) ELISA Kit is specifically designed for the precise measurement of RCN2 levels in human serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, guaranteeing accurate and reproducible results, thus making it an excellent choice for a wide range of research purposes.Reticulocalbin-2 is a vital protein involved in various cellular processes, including calcium binding and protein folding.
Dysregulation of RCN2 has been linked to conditions such as cancer, diabetes, and neurodegenerative disorders, highlighting its importance as a biomarker for studying these diseases and potentially developing targeted therapies.With the Human Reticulocalbin-2 (RCN2) ELISA Kit, researchers can confidently assess RCN2 levels in biological samples, furthering our understanding of its role in health and disease.
Product Name:
Human Reticulocalbin 2 (RCN2) ELISA Kit
Product Code:
HUDL02516
Size:
96 Assays
Target:
RCN2
Synonyms:
E6BP, ERC-55, ERC55, Endoplasmic Reticulum Calcium-Binding Protein 55kD, E6-binding protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
