Human RET (Proto-oncogene tyrosine-protein kinase receptor Ret) ELISA Kit
The Human RET (Proto-oncogene tyrosine-protein kinase receptor Ret) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of the RET protein levels in human biological samples. The Ret receptor is a critical component involved in mediating signaling pathways that regulate cell proliferation, differentiation, and survival.
As a proto-oncogene, RET plays a significant role in various cellular activities and is associated with developmental processes, neuronal function, and tumorigenesis. This ELISA kit provides researchers with a reliable tool to assess RET protein levels, offering valuable insights into signaling cascades, cellular growth, and potential associations with oncogenic activities. By accurately quantifying RET expression levels, researchers can investigate the involvement of the RET pathway in normal physiological functions and disease states, particularly in cancer development and progression. The Human RET ELISA Kit features exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit offers robust performance and ease of use, making it an ideal choice for research studies, biomarker investigations, and drug development targeting the RET signaling pathway in human samples.
Product Name:
Human RET (Proto-oncogene tyrosine-protein kinase receptor Ret) ELISA Kit
SKU:
AEES00653
Target:
Human RET (Proto-oncogene tyrosine-protein kinase receptor Ret)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RET. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RET and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RET, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RET. You can calculate the concentration of Human RET in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
92-105
90-106
Average (%)
102
97
97
1:4
Range (%)
91-107
91-105
93-106
Average (%)
98
98
99
1:8
Range (%)
97-111
88-100
89-99
Average (%)
103
93
94
1:16
Range (%)
93-106
94-105
89-103
Average (%)
101
100
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.92
13.44
48.09
4.58
14.53
43.55
Standard deviation
0.28
0.64
2.14
0.3
0.62
1.65
C V (%)
5.69
4.74
4.46
6.6
4.24
3.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human RET concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RET in samples. No significant cross-reactivity or interference between Human RET and analogues was observed.
