The Human REPIN1 (Replication Initiator 1) CLIA Kit is a cutting-edge assay designed for the precise quantification of REPIN1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of research applications.REPIN1 is a key protein involved in DNA replication initiation, playing a crucial role in cell division and proliferation.
Dysregulation of REPIN1 has been linked to various diseases, including cancer and genetic disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its advanced technology and superior performance, the Human REPIN1 CLIA Kit is an invaluable tool for researchers seeking to investigate the role of REPIN1 in health and disease, offering reliable data and insights for groundbreaking discoveries.
Product Name:
Human REPIN1 (Replication Initiator 1) CLIA Kit
SKU:
HUES00982
Target:
Human REPIN1 (Replication Initiator 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human REPIN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REPIN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REPIN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human REPIN1. You can calculate the concentration of Human REPIN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
100-118
91-104
Average (%)
104
108
99
1:4
Range (%)
91-102
86-98
97-113
Average (%)
97
92
103
1:8
Range (%)
95-109
97-115
91-104
Average (%)
101
105
98
1:16
Range (%)
88-102
96-110
95-110
Average (%)
94
102
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
96-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
203.73
473.54
1490.81
195.05
429.53
1583.76
Standard deviation
22.23
54.65
163.99
17.5
32.77
133.99
C V (%)
10.91
11.54
11.0
8.97
7.63
8.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human REPIN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human REPIN1 in samples. No significant cross-reactivity or interference between Human REPIN1 and analogues was observed.
