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Human Relaxin/Insulin Like Family Peptide Receptor 3 (RXFP3) ELISA Kit

SKU:
HUDL02575
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9NSD7
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GPCR135,RLN3R1,SALPR,RXFPR3,Relaxin 3 Receptor 1,G-protein coupled receptor GPCR135,Somatostatin- and angiotensin-like peptide receptor
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Relaxin/Insulin Like Family Peptide Receptor 3 (RXFP3) ELISA Kit

The Human Relaxin/Insulin-Like Family Peptide Receptor 3 (RXFP3) ELISA Kit is specifically designed for the accurate detection of RXFP3 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.RXFP3 is a key receptor in the relaxin/insulin-like family peptide system, playing a crucial role in regulating various physiological processes such as reproductive functions, cardiovascular health, and inflammation.

Abnormalities in RXFP3 signaling have been linked to several diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its user-friendly design and robust performance, the Human RXFP3 ELISA Kit is an essential tool for researchers and clinicians seeking to better understand the role of RXFP3 in health and disease.

Product Name:Human Relaxin/Insulin Like Family Peptide Receptor 3 (RXFP3) ELISA Kit
Product Code:HUDL02575
Size:96 Assays
Target:RXFP3
Synonyms:GPCR135, RLN3R1, SALPR, RXFPR3, Relaxin 3 Receptor 1, G-protein coupled receptor GPCR135, Somatostatin- and angiotensin-like peptide receptor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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