Human Regenerating Islet Derived Protein 1 Alpha (REG1a) ELISA Kit (HUDL02517)
SKU:
HUDL02517
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05451
Sensitivity:
27.6pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
REG,PSP,ICRF,PSPS1,PTP,Islet cells regeneration factor,Islet of Langerhans regenerating protein,Lithostathine 1 Alpha,Pancreatic Stone Protein,Pancreatic Thread Protein
Human Regenerating Islet Derived Protein 1 Alpha (REG1a) ELISA Kit
The Human Regenerating Islet-Derived Protein 1 Alpha (REG1A) ELISA Kit is designed for the accurate detection of REG1A levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.REG1A is a key protein involved in the regeneration of islet cells in the pancreas, playing a vital role in the maintenance of glucose homeostasis and insulin production.
Dysregulation of REG1A has been associated with conditions such as diabetes and pancreatic disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human REG1A ELISA Kit offers researchers a powerful tool for studying the role of REG1A in pancreatic health and disease, providing valuable insights for the development of novel treatments and interventions in the field of diabetes and pancreatic disorders.
Product Name:
Human Regenerating Islet Derived Protein 1 Alpha (REG1a) ELISA Kit
Product Code:
HUDL02517
Size:
96 Assays
Target:
REG1a
Synonyms:
REG, PSP, ICRF, PSPS1, PTP, Islet cells regeneration factor, Islet of Langerhans regenerating protein, Lithostathine 1 Alpha, Pancreatic Stone Protein, Pancreatic Thread Protein
Detection Method:
Sandwich
Range:
62.5-4,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
