Human Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit
The Human Receptor Interacting Serine/Threonine Kinase 3 (RIPK3) ELISA Kit is a specialized assay designed for the accurate measurement of RIPK3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for precise and reproducible results, making it a valuable tool for various research applications.RIPK3 is a critical kinase involved in cell death pathways, inflammation, and immune responses. Dysregulation of RIPK3 has been linked to various diseases including inflammatory disorders, autoimmune conditions, and neurodegenerative diseases.
Therefore, studying RIPK3 levels can provide valuable insights into disease mechanisms and potential therapeutic strategies.By utilizing the Human RIPK3 ELISA Kit, researchers can accurately quantify RIPK3 levels in samples, enabling them to investigate the role of RIPK3 in disease pathogenesis and identify novel therapeutic targets. This kit is a reliable and efficient solution for studying RIPK3 biology and its implications in various disease conditions.
Product Name:
Human Receptor Interacting Serine Threonine Kinase 3 (RIPK3) ELISA Kit
Product Code:
HUDL02540
Size:
96 Assays
Synonyms:
RIP3, RIP-like protein kinase 3, Receptor-interacting protein 3
Detection Method:
Sandwich
Range:
31.2-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
