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Human Receptor Activity Modifying Protein 2 (RAMP2) ELISA Kit

SKU:
HUDL02496
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O60895
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
Calcitonin-receptor-like receptor activity-modifying protein 2,Receptor(G Protein-Coupled)Activity Modifying Protein 2,Receptor(Calcitonin)Activity Modifying Protein 2
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Receptor Activity Modifying Protein 2 (RAMP2) ELISA Kit

The Human Receptor Activity Modifying Protein 2 (RAMP2) ELISA Kit is specifically designed to accurately detect levels of RAMP2 in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it perfect for a variety of research applications.RAMP2 is a critical protein that plays a crucial role in regulating receptor activity and signal transduction pathways. It is involved in various physiological processes, including cardiovascular function, blood pressure regulation, and pain sensation.

Dysregulation of RAMP2 has been linked to diseases such as hypertension, migraines, and inflammatory conditions, making it an important biomarker for studies and potential therapeutic developments.Overall, the Human Receptor Activity Modifying Protein 2 (RAMP2) ELISA Kit offers a valuable tool for researchers looking to investigate the role of RAMP2 in health and disease, providing accurate and detailed insights into its function and potential implications for various conditions.

Product Name:Human Receptor Activity Modifying Protein 2 (RAMP2) ELISA Kit
Product Code:HUDL02496
Size:96 Assays
Target:RAMP2
Synonyms:Calcitonin-receptor-like receptor activity-modifying protein 2, Receptor(G Protein-Coupled)Activity Modifying Protein 2, Receptor(Calcitonin)Activity Modifying Protein 2
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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