Human RDH11 ELISA Kit (HUFI06863)- High Sensitivity

Product Name:	Human RDH11 (Retinol dehydrogenase 11)ELISA Kit
Product Code:	HUFI06863
Size:	96 Assays
Alias:	RDH11 ELISA Kit, Retinol dehydrogenase 11 ELISA Kit, Androgen-regulated short-chain dehydrogenase/reductase 1 ELISA Kit, HCV core-binding protein HCBP12 ELISA Kit, Prostate short-chain dehydrogenase/reductase 1 ELISA Kit, Retinal reductase 1 ELISA Kit, RalR1 ELISA Kit, ARSDR1 ELISA Kit, PSDR1 ELISA Kit, SDR7C1 ELISA Kit, CGI-82 ELISA Kit, Short chain dehydrogenase/reductase family 7C member 1 ELISA Kit
Detection method:	Sandwich ELISA, Double Antibody
Application:	This immunoassay kit allows for the in vitro quantitative determination of Human RDH11 (Retinol dehydrogenase 11)ELISA Kit concentrations in serum plasma and other biological fluids.
Sensitivity:	< 46.875pg/ml
Range:	78.125-5000pg/ml
Storage:	4°C for 6 months
Note:	For Research Use Only

Recovery:

Matrices listed below were spiked with certain level of Human RDH11 (Retinol dehydrogenase 11)ELISA Kit and the recovery rates were calculated by comparing the measured value to the expected amount of Human RDH11 (Retinol dehydrogenase 11)ELISA Kit in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	89-100	94
EDTA plasma(n=5)	87-98	92
heparin plasma(n=5)	86-99	92

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human RDH11 (Retinol dehydrogenase 11)ELISA Kit and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-92%	90-102%	92-103%
EDTA plasma(n=5)	90-101%	83-97%	85-99%
UFH plasma(n=5)	80-100%	80-99%	83-97%

CV(%):

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Component	Quantity	Storage
ELISA Microplate (Dismountable)	8×12 strips	4°C for 6 months
Lyophilized Standard	2	4°C/-20°C
Sample/Standard Dilution Buffer	20ml	4°C
Biotin-labeled Antibody(Concentrated)	120ul	4°C (Protect from light)
Antibody Dilution Buffer	10ml	4°C
HRP-Streptavidin Conjugate(SABC)	120ul	4°C (Protect from light)
SABC Dilution Buffer	10ml	4°C
TMB Substrate	10ml	4°C (Protect from light)
Stop Solution	10ml	4°C
Wash Buffer(25X)	30ml	4°C
Plate Sealer	5	-

Other materials and equipment required:

Microplate reader with 450 nm wavelength filter
Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
Incubator
Deionized or distilled water
Absorbent paper
Buffer resevoir

UniProt Protein Function:	RDH11: Exhibits an oxidoreductive catalytic activity towards retinoids. Most efficient as an NADPH-dependent retinal reductase. Displays high activity towards 9-cis and all-trans-retinol. Also involved in the metabolism of short-chain aldehydes. No steroid dehydrogenase activity detected. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase; EC 1.1.1.300 Chromosomal Location of Human Ortholog: 14q24.1 Cellular Component: endoplasmic reticulum membrane; photoreceptor inner segment; integral to membrane; intracellular Molecular Function:retinol dehydrogenase activity Biological Process: phototransduction, visible light; retinal metabolic process; retinol metabolic process; adaptation of rhodopsin mediated signaling; retinoid metabolic process Disease: Retinal Dystrophy, Juvenile Cataracts, And Short Stature Syndrome
NCBI Summary:	The protein encoded by this gene is an NADPH-dependent retinal reductase and a short-chain dehydrogenase/reductase. The encoded protein has no steroid dehydrogenase activity. [provided by RefSeq, Nov 2011]
UniProt Code:	Q8TC12
NCBI GenInfo Identifier:	90110515
NCBI Gene ID:	51109
NCBI Accession:	ABD90542.1
UniProt Secondary Accession:	Q8TC12,Q6IAH5, Q9NRW0, Q9Y391, A6NDK3, A8K062, B2RB26 B4DDW0,
UniProt Related Accession:	Q8TC12
Molecular Weight:	35,386 Da
NCBI Full Name:	retinol dehydrogenase 11, partial
NCBI Synonym Full Names:	retinol dehydrogenase 11 (all-trans/9-cis/11-cis)
NCBI Official Symbol:	RDH11
NCBI Official Synonym Symbols:	MDT1; CGI82; PSDR1; RALR1; SCALD; ARSDR1; HCBP12; SDR7C1
NCBI Protein Information:	retinol dehydrogenase 11; retinal reductase 1; HCV core-binding protein HCBP12; prostate short-chain dehydrogenase reductase 1; prostate short-chain dehydrogenase/reductase 1; short chain dehydrogenase/reductase family 7C, member 1; androgen-regulated short-chain dehydrogenase/reductase 1
UniProt Protein Name:	Retinol dehydrogenase 11
UniProt Synonym Protein Names:	Androgen-regulated short-chain dehydrogenase/reductase 1; HCV core-binding protein HCBP12; Prostate short-chain dehydrogenase/reductase 1; Retinal reductase 1
Protein Family:	Retinol dehydrogenase
UniProt Gene Name:	RDH11
UniProt Entry Name:	RDH11_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37 °C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

Step	Protocol
1.	Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.	Aliquot 0.1ml standard solutions into the standard wells.
3.	Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.	Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.	Seal the plate with a cover and incubate at 37 °C for 90 min.
6.	Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.	Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.	Seal the plate with a cover and incubate at 37 °C for 60 min.
9.	Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.	Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37 °C for 30 min.
11.	Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.	Add 90 µL of TMB substrate into each well, cover the plate and incubate at 37 °C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.	Add 50 µL of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14.	Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type	Protocol
Serum	If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.
Plasma	Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal Fluid	Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatant	Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysates	Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenates	The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysates	Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast Milk	Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

Antibodies

Anti-RDH11 Antibody (CAB18454)

RDH11 Antibody (PACO11785)

RDH11 Antibody (PACO16960)

RDH11 Antibody (PACO29436)

RDH11 Antibody, HRP conjugated (PACO29437)