Human RDBP Recombinant Protein (RPPB4434)
- SKU:
- RPPB4434
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P18615
Description
| Product Name: | Human RDBP Recombinant Protein |
| Product Code: | RPPB4434 |
| Size: | 25µg |
| Species: | Human |
| Target: | RDBP |
| Synonyms: | RD RNA binding protein, NELF-E, RD, D6S45, RDP, Major Histocompatibility Complex Gene RD, Negative Elongation Factor Polypeptide E, nuclear protein, RDBP. |
| Source: | Escherichia Coli |
| Physical Appearance: | The RDBP is supplied as a sterile filtered clear solution. |
| Formulation: | The RDBP protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 2mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | RDBP purity was found to be greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL SEQPVMDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE KGPVPTFQPF QRSISADDDL QESSRRPQRK SLYESFVSSS DRLRELGPDG EEAEGPGAGD GPPRSFDWGY EERSGAHSSA SPPRSRSRDR SHERNRDRDR DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR DREGPFRRSD SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV QNSPKGCHRD KRTQIVYSDD VYKENLVDGF |
RDBP is a putative RNA binding protein. RDBP protein is one of the five components of the multisubunit NELF complex which collaborates with DSIF to repress RNA polymerase II elongation. Control of transcription elongation needs a complex interaction between positive transcription elongation factor b and negative transcription elongation factors, DSIF and NELF. DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. RDBP has a functional RNA-binding domain, whose mutations impair transcription repression without affecting known protein-protein interactions.
RDBP produced in E.Coli is a single, non-glycosylated polypeptide chain containing 400 amino acids (1-380a.a.) and having a molecular mass of 45.4 kDa. RDBP is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RDBP: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Belongs to the RRM NELF-E family. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Mitochondrial Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: nucleoplasm; mitochondrion; nucleus Molecular Function:protein binding; RNA binding; nucleotide binding Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression |
| NCBI Summary: | The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P18615 |
| NCBI GenInfo Identifier: | 1350554 |
| NCBI Gene ID: | 7936 |
| NCBI Accession: | P18615.3 |
| UniProt Related Accession: | P18615 |
| Molecular Weight: | 43kDa |
| NCBI Full Name: | Negative elongation factor E |
| NCBI Synonym Full Names: | negative elongation factor complex member E |
| NCBI Official Symbol: | NELFE�� |
| NCBI Official Synonym Symbols: | RD; RDP; RDBP; D6S45; NELF-E�� |
| NCBI Protein Information: | negative elongation factor E |
| UniProt Protein Name: | Negative elongation factor E |
| UniProt Synonym Protein Names: | RNA-binding protein RD |
| UniProt Gene Name: | NELFE�� |
| UniProt Entry Name: | NELFE_HUMAN |
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