Human RBP-4 PharmaGenie ELISA - Kit Information
RBP4 (retinol binding protein-4) is an adipocyte-secreted molecule that is elevated in the serum before the development of frank diabetes. In obesity and type 2 diabetes, expression of the GLUT4 glucose transporter is decreased selectively in adipocytes. Adipose-specific Glut4 knockout mice show insulin resistance secondarily in muscle and liver. Recent studies have shown that serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes. RBP4 levels are normalized by rosiglitazone, an insulin-sensitizing drug. Transgenic overexpression of human RBP4 or injection of recombinant RBP4 in normal mice causes insulin resistance. Conversely, genetic deletion of RBP4 enhances insulin sensitivity. Fenretinide, a synthetic retinoid that increases urinary excretion of RBP4, normalizes serum RBP4 levels and improves insulin resistance and glucose intolerance in mice with obesity induced by a high-fat diet. Increasing serum RBP4 induces hepatic expression of the gluconeogenic enzyme phosphoenolpyruvate carboxykinase (PEPCK) and impairs insulin signalling in muscle. Thus, RBP4 is an adipocyte-derived 'signal' that may contribute to the pathogenesis of type 2 diabetes. Lowering RBP4 could be a new strategy for treating type 2 diabetes. Thus, RBP4 appears to identify insulin resistance and associated cardiovascular risk factors in subjects with varied clinical presentations.
Human RBP-4 PharmaGenie ELISA - Kit Data
| Product SKU: | SBRS0118 |
| Size: | 96T |
| Application: | This kit detects RBP4 (183aa) and all other active forms including RBP4-182,181,179,176. Cross Reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC. |
| Uniprot: | P02753 |
| Gene ID: | 5950 |
| Gene Names: | PRO2222 / RBP4 |
| Synonyms: | Retinol-binding protein 4 (Plasma retinol-binding protein) (PRBP) (RBP) [Cleaved into: Plasma retinol-binding protein(1-182) Plasma retinol-binding protein(1-181) Plasma retinol-binding protein(1-179) Plasma retinol-binding protein(1-176)] |
| Target Species: | Human, Mouse, Rat |
| Compatible Sample Types: | Cell Culture Supernatants and Serum |
| Design Principle: | Competition-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.1-1,000 ng/ml |
| Sensitivity: | 460 pg/ml |
| Recommended Dilution: | Human: 4X, Mouse: 2X |
| Storage/Stability: | Standard, Biotinylated Retinol Binding Protein 4 peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge. |
The Assay Genie Human RBP-4 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.
