Human RBM17 Recombinant Protein (RPPB4422)
- SKU:
- RPPB4422
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q96I25
Description
| Product Name: | Human RBM17 Recombinant Protein |
| Product Code: | RPPB4422 |
| Size: | 10µg |
| Species: | Human |
| Target: | RBM17 |
| Synonyms: | RNA Binding Motif Protein 17, SPF45, Splicing Factor 45kDa, RNA-Binding Motif Protein 17, Splicing Factor 45, 45 kDa-splicing factor, RBM17. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | RBM17 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSLYDDLGVE TSDSKTEGWS KNFKLLQSQL QVKKAALTQA KSQRTKQSTV LAPVIDLKRG GSSDDRQIVD TPPHVAAGLK DPVPSGFSAG EVLIPLADEY DPMFPNDYEK VVKRQREERQ RQRELERQKE IEEREKRRKD RHEASGFARR PDPDSDEDED YERERRKRSM GGAAIAPPTS LVEKDKELPR DFPYEEDSRP RSQSSKAAIP PPVYEEQDRP RSPTGPSNSF LANMGGTVAH KIMQKYGFRE GQGLGKHEQG LSTALSVEKT SKRGGKIIVG DATEKDASKK SDSNPLTEIL KCPTKVVLLR NMVGAGEVDE DLEVETKEEC EKYGKVGKCV IFEIPGAPDD EAVRIFLEFE RVESAIKAVV DLNGRYFGGR VVKACFYNLD KFRVLDLAEQ V |
RNA binding motif protein 17 (RBM17) is a splice factor which binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the 2nd catalytic step. The RBM17 protein is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. RBM17 protein also stimulates the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The ensuing frameshift leads to sickle cell anemia.
RBM17 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 421 amino acids (1-401 a.a) and having a molecular mass of 47.1kDa.RBM17 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RBM17: Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia. |
| UniProt Protein Details: | Protein type:RNA splicing; RNA-binding; Spliceosome Chromosomal Location of Human Ortholog: 10p15.1 Cellular Component: nucleoplasm; spliceosome Molecular Function:protein binding; RNA binding; nucleotide binding Biological Process: alternative nuclear mRNA splicing, via spliceosome |
| NCBI Summary: | This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15. [provided by RefSeq, Mar 2009] |
| UniProt Code: | Q96I25 |
| NCBI GenInfo Identifier: | 34925383 |
| NCBI Gene ID: | 84991 |
| NCBI Accession: | Q96I25.1 |
| UniProt Secondary Accession: | Q96I25,Q96GY6, |
| UniProt Related Accession: | Q96I25 |
| Molecular Weight: | 401 |
| NCBI Full Name: | Splicing factor 45 |
| NCBI Synonym Full Names: | RNA binding motif protein 17 |
| NCBI Official Symbol: | RBM17�� |
| NCBI Official Synonym Symbols: | SPF45�� |
| NCBI Protein Information: | splicing factor 45; splicing factor 45kDa; 45 kDa-splicing factor; RNA-binding motif protein 17 |
| UniProt Protein Name: | Splicing factor 45 |
| UniProt Synonym Protein Names: | 45 kDa-splicing factor; RNA-binding motif protein 17 |
| Protein Family: | Splicing factor |
| UniProt Gene Name: | RBM17�� |
| UniProt Entry Name: | SPF45_HUMAN |
