Human Ras Association Domain Containing Protein 1 (RASSF1) ELISA Kit
The Human RASSF1 (Ras association domain-containing protein 1) ELISA Kit is a highly reliable assay designed for the accurate measurement of RASSF1 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and reproducible results, making it an essential tool for researchers in various fields.RASSF1 is a protein that plays a crucial role in cellular processes such as cell cycle regulation, apoptosis, and tumor suppression. Dysregulation of RASSF1 has been implicated in various diseases including cancer, making it a valuable biomarker for studying disease progression and potential therapeutic interventions.
By utilizing the Human RASSF1 ELISA Kit, researchers can accurately quantify RASSF1 protein levels in human samples, providing valuable insights into disease mechanisms and potential treatment strategies. This kit is user-friendly, cost-effective, and suitable for high-throughput applications, making it an indispensable tool for advancing research in the field.
Product Name:
Human Ras Association Domain Containing Protein 1 (RASSF1) ELISA Kit
Product Code:
HUDL02507
Size:
96 Assays
Target:
RASSF1
Synonyms:
123F2, NORE2A, RASSF1A, RDA32, REH3P21
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
