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Human RalA Binding Protein 1 (RALBP1) ELISA Kit

SKU:
HUDL02495
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q15311
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
RIP,RIP1,RLIP76,76 kDa Ral-interacting protein,Ral-interacting protein 1,Dinitrophenyl S-glutathione ATPase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human RalA Binding Protein 1 (RALBP1) ELISA Kit

The Human RALBP1 (RALA Binding Protein 1) ELISA Kit is a highly sensitive and reliable assay designed for the accurate detection of RALBP1 levels in human serum, plasma, and cell culture supernatants. This kit offers high specificity, ensuring reproducible results for a variety of research applications.RALBP1 is a key protein involved in RALA signaling pathways, playing a crucial role in cellular processes such as endocytosis, membrane trafficking, and cell migration. Dysregulation of RALBP1 has been linked to various diseases, including cancer, autoimmune disorders, and neurological conditions, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.

With its high performance and ease of use, the Human RALBP1 ELISA Kit is an essential tool for researchers looking to investigate the role of RALBP1 in health and disease, providing valuable insights into novel treatment strategies and diagnostic approaches.

Product Name:Human RalA Binding Protein 1 (RALBP1) ELISA Kit
Product Code:HUDL02495
Size:96 Assays
Target:RALBP1
Synonyms:RIP, RIP1, RLIP76, 76 kDa Ral-interacting protein, Ral-interacting protein 1, Dinitrophenyl S-glutathione ATPase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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