The Human RAD23 Homolog B (RAD23B) ELISA Kit is specifically designed for the precise measurement of RAD23B levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and consistent results, making it suitable for a wide variety of research applications.RAD23B is a protein that plays a critical role in DNA repair and ubiquitin-mediated proteolysis, important processes for maintaining genomic stability and regulating protein levels within cells.
Dysregulation of RAD23B has been linked to various diseases including cancer, neurodegenerative disorders, and other conditions, making it a valuable biomarker for studying these diseases and developing potential therapies.Overall, the Human RAD23 Homolog B (RAD23B) ELISA Kit is an essential tool for researchers looking to study the role of RAD23B in various diseases and molecular pathways, providing reliable and valuable data for advancing scientific knowledge and potential therapeutic developments.
Product Name:
Human RAD23 Homolog B (RAD23B) ELISA Kit
Product Code:
HUDL02490
Size:
96 Assays
Target:
RAD23B
Synonyms:
HHR23B, P58, HR23B, XP-C Repair Complementing Protein, XP-C Repair Complementing Complex 58 kDa, UV excision repair protein RAD23 homolog B
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
