Human RAB5C, Member RAS Oncogene Family (RAB5C) ELISA Kit
The Human RAB5C (Member of RAS Oncogene Family) ELISA Kit is designed for the accurate quantification of RAB5C levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.RAB5C is a member of the RAS oncogene family and plays a crucial role in intracellular membrane trafficking and signal transduction. Dysregulation of RAB5C has been implicated in various diseases, including cancer, neurodegenerative disorders, and infectious diseases.
The ability to accurately measure RAB5C levels can provide valuable insights into disease pathogenesis and identify potential therapeutic targets.The Human RAB5C ELISA Kit is an essential tool for researchers studying the molecular mechanisms of disease and exploring novel therapeutic strategies. Its reliable performance and ease of use make it a valuable asset in the field of biomedical research.
Product Name:
Human RAB5C, Member RAS Oncogene Family (RAB5C) ELISA Kit
Product Code:
HUDL02485
Size:
96 Assays
Target:
RAB5C
Synonyms:
RABL, RAB5CL, Ras-related protein Rab-5C
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
