Human RAB35 Recombinant Protein (RPPB4362)
- SKU:
- RPPB4362
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q15286
Description
| Product Name: | Human RAB35 Recombinant Protein |
| Product Code: | RPPB4362 |
| Size: | 10µg |
| Species: | Human |
| Target: | RAB35 |
| Synonyms: | Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C, RAB35, RAB1C, RAY. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | RAB35 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MARDYDHLFK LLIIGDSGVG KSSLLLRFAD NTFSGSYITT IGVDFKIRTV EINGEKVKLQ IWDTAGQERF RTITSTYYRG THGVIVVYDV TSAESFVNVK RWLHEINQNC DDVCRILVGN KNDDPERKVV ETEDAYKFAG QMGIQLFETS AKENVNVEEM FNCITELVLR AKKDNLAKQQ QQQQNDVVKL TKNSKRKKRC C |
Ras-related protein Rab-35 (RAB35) is restricted to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. Inhibition of Rab35 function leads to the accumulation of endocytic markers on numerous cytoplasmic vacuoles in cells that failed cytokinesis, which is consistent with a central requirement for Rab35-regulated recycling during cell division.
RAB35 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 221 amino acids (1-201 a.a.) and having a molecular mass of 25.2kDa.RAB35 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RAB35: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. Interacts with DENND1A and DENND1B in a nucleotide- dependent manner. The low binding to DENND1C is nucleotide- independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:G protein; G protein, monomeric, Rab; G protein, monomeric Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: cell projection membrane; clathrin-coated endocytic vesicle; coated pit; endoplasmic reticulum membrane; endosome membrane; Golgi membrane; intercellular bridge; plasma membrane Molecular Function:GDP binding; GTP binding; GTPase activity; phosphatidylinositol-4,5-bisphosphate binding; protein binding Biological Process: antigen processing and presentation; cytokinesis; endocytic recycling; endosome transport; ER to Golgi vesicle-mediated transport; neurite development; plasma membrane to endosome transport; protein localization |
| UniProt Code: | Q15286 |
| NCBI GenInfo Identifier: | 3024525 |
| NCBI Gene ID: | 11021 |
| NCBI Accession: | Q15286.1 |
| UniProt Secondary Accession: | Q15286,B2R6E0, B4E390, |
| UniProt Related Accession: | Q15286 |
| Molecular Weight: | 17,058 Da |
| NCBI Full Name: | Ras-related protein Rab-35 |
| NCBI Synonym Full Names: | RAB35, member RAS oncogene family |
| NCBI Official Symbol: | RAB35�� |
| NCBI Official Synonym Symbols: | RAY; H-ray; RAB1C�� |
| NCBI Protein Information: | ras-related protein Rab-35 |
| UniProt Protein Name: | Ras-related protein Rab-35 |
| UniProt Synonym Protein Names: | GTP-binding protein RAY; Ras-related protein Rab-1C |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAB35�� |
| UniProt Entry Name: | RAB35_HUMAN |
