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Human RAB1A, Member RAS Oncogene Family (RAB1A) ELISA Kit

SKU:
HUDL02482
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P62820
Sensitivity:
0.067ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
RAB1,YPT1,Ras-related protein Rab-1A,YPT1-related protein
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Human RAB1A, Member RAS Oncogene Family (RAB1A) ELISA Kit

The Human Rab1A (Member of Ras Oncogene Family) ELISA Kit is specifically designed for the accurate measurement of Rab1A levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Rab1A is a key protein in the Ras oncogene family, playing a crucial role in intracellular trafficking and vesicular transport. Dysregulation of Rab1A has been implicated in various diseases, including cancer, neurodegenerative disorders, and metabolic diseases.

Therefore, the measurement of Rab1A levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its advanced technology and reliable performance, the Human Rab1A ELISA Kit is a valuable tool for researchers studying Rab1A biology and its implications in various pathological conditions. Order yours today and accelerate your research with confidence.

Product Name:Human RAB1A, Member RAS Oncogene Family (RAB1A) ELISA Kit
Product Code:HUDL02482
Size:96 Assays
Target:RAB1A
Synonyms:RAB1, YPT1, Ras-related protein Rab-1A, YPT1-related protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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