Human Quinoid Dihydropteridine Reductase (QDPR) ELISA Kit
The Human Quinoid Dihydropteridine Reductase (QDPR) ELISA Kit is a powerful tool for precise measurement of QDPR levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides accurate and consistent results, making it suitable for a diverse array of research purposes.QDPR is a vital enzyme involved in the biosynthesis of tetrahydrobiopterin, a crucial cofactor for various enzymes including phenylalanine hydroxylase and tyrosine hydroxylase.
Dysregulation of QDPR has been associated with a range of disorders such as phenylketonuria and neurotransmitter deficiencies, underscoring its importance as a biomarker for these conditions and as a target for potential therapeutic interventions.Overall, the Human QDPR ELISA Kit offers researchers a reliable and efficient means to assess QDPR levels and gain insights into its role in health and disease.
Product Name:
Human Quinoid Dihydropteridine Reductase (QDPR) ELISA Kit
Product Code:
HUDL02479
Size:
96 Assays
Target:
QDPR
Synonyms:
DHPR, PKU2, SDR33C1, HDHPR, 6,7-Dihydropteridine Reductase, Short Chain Dehydrogenase/Reductase Family 33C,Member 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
