Human PYGL (Glycogen Phosphorylase, Liver) ELISA Kit
The Human PYGL (Glycogen Phosphorylase, Liver) ELISA Kit is a specialized assay meticulously tailored for the precise measurement of PYGL levels in human biological samples. PYGL, also known as liver glycogen phosphorylase, is a crucial enzyme playing a central role in glycogen metabolism by catalyzing the breakdown of glycogen into glucose-1-phosphate in the liver. This enzymatic process is vital for regulating blood glucose levels and providing energy during fasting or exercise. This ELISA kit offers researchers a powerful tool to quantify PYGL expression, shedding light on its involvement in glycogenolysis, glucose homeostasis, and metabolic functions.
By accurately assessing PYGL levels, researchers can deepen their understanding of glycogen metabolism regulation, hepatic energy utilization, and the pathophysiology of metabolic disorders such as diabetes and glycogen storage diseases. The Human PYGL ELISA Kit provides outstanding sensitivity and specificity, ensuring precise and reproducible results. With robust performance and user-friendly protocols, this kit facilitates comprehensive investigations into the role of PYGL in liver function, metabolic pathways, and metabolic disorders, making it an invaluable asset for scientific research and clinical studies.
Product Name:
Human PYGL (Glycogen Phosphorylase, Liver) ELISA Kit
SKU:
AEES00699
Target:
Human PYGL (Glycogen Phosphorylase, Liver)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PYGL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PYGL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PYGL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PYGL. You can calculate the concentration of Human PYGL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
90-103
86-96
Average (%)
103
98
91
1:4
Range (%)
88-100
82-97
87-99
Average (%)
94
89
94
1:8
Range (%)
94-106
88-98
84-95
Average (%)
100
93
89
1:16
Range (%)
91-105
87-102
84-99
Average (%)
99
93
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
90-100
95
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
0.89
4.29
0.52
0.9
4.49
Standard deviation
0.03
0.05
0.2
0.04
0.04
0.19
C V (%)
5.88
5.62
4.66
7.69
4.44
4.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PYGL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PYGL in samples. No significant cross-reactivity or interference between Human PYGL and analogues was observed.
