Human Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14) ELISA Kit
The Human Purinergic Receptor P2Y G Protein Coupled 14 (P2RY14) ELISA Kit is a reliable and accurate tool for detecting P2RY14 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.P2RY14 is a key receptor involved in purinergic signaling, which plays a critical role in various physiological processes including immune responses, inflammation, and neurotransmission. Dysregulation of P2RY14 has been linked to conditions such as autoimmune disorders, inflammatory diseases, and neurological disorders, making it an important target for research and potential therapeutic interventions.
By accurately measuring P2RY14 levels, researchers can gain valuable insights into the role of purinergic signaling in health and disease, paving the way for the development of novel treatments and interventions. The Human Purinergic Receptor P2Y G Protein Coupled 14 (P2RY14) ELISA Kit is a valuable tool for advancing scientific knowledge and driving future breakthroughs in medical research.
Product Name:
Human Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
