The Human PTX3/TSG-14 (Pentraxin 3) ELISA Kit is a purpose-built assay designed for the quantitative measurement of Pentraxin 3 protein levels in various human biological samples. Pentraxin 3 (PTX3) is a critical fluid-phase pattern recognition receptor associated with diverse inflammatory processes and immune responses. It serves as a valuable diagnostic marker in various conditions, including infectious diseases, vascular disorders, and inflammatory responses.
Accurate quantification of PTX3 is essential for elucidating its role in various pathological conditions and developing precise therapeutic interventions. Our ELISA kit is meticulously developed to provide outstanding sensitivity and specificity, enabling accurate and reproducible results for the quantitation of PTX3 levels. Manufactured under strict quality control measures, this kit ensures robust and reliable performance, offering a scientific tool of choice for both research investigations and clinical applications. Rely on the Human PTX3/TSG-14 ELISA Kit to achieve precise and dependable quantification of this vital biomarker for your scientific inquiries.
Product Name:
Human PTX 3/TSG-14 (Pentraxin 3) ELISA Kit
SKU:
AEES00117
Target:
Human PTX 3/TSG-14 (Pentraxin 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.12 pg/mL
Detection range:
6.86-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTX3/TSG-14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTX3/TSG-14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTX3/TSG-14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PTX3/TSG-14. You can calculate the concentration of Human PTX3/TSG-14 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
95-107
92-108
Average (%)
99
101
99
1:4
Range (%)
91-105
94-108
93-104
Average (%)
98
99
98
1:8
Range (%)
96-109
94-110
90-106
Average (%)
102
101
97
1:16
Range (%)
94-105
95-107
88-102
Average (%)
100
101
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
93
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
86-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
230.55
565.35
2010.46
209.0
617.46
1987.07
Standard deviation
13.63
24.99
96.7
13.63
29.64
104.52
C V (%)
5.91
4.42
4.81
6.52
4.8
5.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PTX3/TSG-14 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PTX3/TSG-14 in samples. No significant cross-reactivity or interference between Human PTX3/TSG-14 and analogues was observed.
