null

Human PSMF1 Recombinant Protein (RPPB2218)

SKU:
RPPB2218
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q92530
Research Area:
Enzymes
€469
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human PSMF1 Recombinant Protein
Product Code:RPPB2218
Size:20µg
Species:Human
Target:PSMF1
Synonyms:Proteasome inhibitor PI31 subunit, hPI31, PSMF1, PI31.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PSMF1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 1mM DTT and 0.1mM PMSF.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAGLEVLFAS AAPAITCRQD ALVCFLHWEV VTHGYCGLGV GDQPGPNDKK SELLPAGWNN NKDLYVLRYE YKDGSRKLLV KAITVESSMI LNVLEYGSQQ VADLTLNLDD YIDAEHLGDF HRTYKNSEEL RSRIVSGIIT PIHEQWEKAN VSSPHREFPP ATAREVDPLR IPPHHPHTSR QPPWCDPLGP FVVGGEDLDP FGPRRGGMIV DPLRSGFPRA LIDPSSGLPN RLPPGAVPPG ARFDPFGPIG TSPPGPNPDH LPPPGYDDMY L

PSMF1 is a protein which hinders the activation of the proteasome by the 11S and 19S regulators. The 26S proteasome is a multicatalytic proteinase complex with an extremely ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is comprised of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits.

PSMF1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 291 amino acids (1-271) and having a molecular mass of 31.9kDa.PSMF1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PSMF1: Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28. Belongs to the proteasome inhibitor PI31 family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: nucleoplasm; proteasome core complex; membrane; endoplasmic reticulum; cytoplasm; cytosol

Molecular Function:protein binding; endopeptidase inhibitor activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis

NCBI Summary:The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q92530
NCBI GenInfo Identifier:134047876
NCBI Gene ID:9491
NCBI Accession:Q92530.2
UniProt Secondary Accession:Q92530,Q9H4I1, A0AVQ9, D3DVW3,
UniProt Related Accession:Q92530
Molecular Weight:29,817 Da
NCBI Full Name:Proteasome inhibitor PI31 subunit
NCBI Synonym Full Names:proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
NCBI Official Symbol:PSMF1��
NCBI Official Synonym Symbols:PI31��
NCBI Protein Information:proteasome inhibitor PI31 subunit; hPI31; proteasome inhibitor hP131 subunit
UniProt Protein Name:Proteasome inhibitor PI31 subunit
Protein Family:Proteasome inhibitor
UniProt Gene Name:PSMF1��
UniProt Entry Name:PSMF1_HUMAN

Related Products