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Human PSMB7 Recombinant Protein (RPPB2210)

SKU:
RPPB2210
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q99436
Research Area:
Enzymes
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Description

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Product Name:Human PSMB7 Recombinant Protein
Product Code:RPPB2210
Size:5µg
Species:Human
Target:PSMB7
Synonyms:Proteasome subunit beta type-7, Macropain chain Z, Multicatalytic endopeptidase complex chain Z, Proteasome subunit Z, PSMB7, Z.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PSMB7 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MTTIAGVVYK DGIVLGADTR ATEGMVVADK NCSKIHFISP NIYCCGAGTA ADTDMTTQLI SSNLELHSLS TGRLPRVVTA NRMLKQMLFR YQGYIGAALV LGGVDVTGPH LYSIYPHGST DKLPYVTMGS GSLAAMAVFE DKFRPDMEEE EAKNLVSEAI AAGIFNDLGS GSNIDLCVIS KNKLDFLRPY TVPNKKGTRL GRYRCEKGTT AVLTEKITPL EIEVLEETVQ TMDTS

Proteasome subunit beta type-7 (PSMB7) belongs to the proteasome B-type family. PSMB7 is a multicatalytic proteinase complex with an extremely ordered ring-shaped 20S core structure. This core structure is comprised of 4 rings of 28 non-identical subunits; 2 rings are comprised of 7 alpha subunits and 2 rings are comprised of 7 beta subunits. Proteasomes are scattered all over eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent manner in a non-lysosomal pathway. The processing of class I MHC peptides is a vital function of the immunoproteasome (which is a modified proteasome).

PSMB7 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 255 amino acids (44-277) and having a molecular mass of 27.6kDa (Molecular size on SDS-PAGE will appear higher).PSMB7 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PSMB7: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. Not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit).
UniProt Protein Details:

Protein type:Protease; Proteasome complex; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 9q34.11-q34.12

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; proteasome complex; proteasome core complex

Molecular Function:protein binding

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway

NCBI Summary:The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14. [provided by RefSeq, Jul 2012]
UniProt Code:Q99436
NCBI GenInfo Identifier:17380263
NCBI Gene ID:5695
NCBI Accession:Q99436.1
UniProt Secondary Accession:Q99436,Q5TBG6, Q96AG8, Q9BWA7, B4E0P1,
UniProt Related Accession:Q99436
Molecular Weight:15,323 Da
NCBI Full Name:Proteasome subunit beta type-7
NCBI Synonym Full Names:proteasome subunit beta 7
NCBI Official Symbol:PSMB7��
NCBI Official Synonym Symbols:Z��
NCBI Protein Information:proteasome subunit beta type-7
UniProt Protein Name:Proteasome subunit beta type-7
UniProt Synonym Protein Names:Macropain chain Z; Multicatalytic endopeptidase complex chain Z; Proteasome subunit Z
Protein Family:Proteasome
UniProt Gene Name:PSMB7��
UniProt Entry Name:PSB7_HUMAN

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