Human PSMA (Prostate specific membrane antigen) ELISA Kit
The Human PSMA (Prostate-Specific Membrane Antigen) ELISA Kit is specifically designed for the accurate detection of PSMA levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring dependable and reproducible results for a variety of research applications.PSMA is a key protein that is primarily expressed in prostate tissue, making it a valuable biomarker for prostate cancer diagnosis and research.
Its overexpression in prostate cancer cells makes it an ideal target for diagnostic and therapeutic purposes. The Human PSMA ELISA Kit provides researchers with a reliable tool to study PSMA levels in various biological samples and further understand its role in prostate cancer development and progression.
Product Name:
Human PSMA (Prostate specific membrane antigen) ELISA Kit
SKU:
HUES03416
Target:
Human PSMA (Prostate specific membrane antigen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
2.81 ng/mL
Detection range:
4.69-300 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PSMA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PSMA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PSMA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PSMA. You can calculate the concentration of Human PSMA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
90-104
98-109
Average (%)
93
98
104
1:4
Range (%)
88-101
80-94
88-100
Average (%)
95
87
94
1:8
Range (%)
89-103
85-99
88-99
Average (%)
97
90
93
1:16
Range (%)
94-104
82-92
87-101
Average (%)
99
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
84-100
91
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
15.17
30.8
142.79
13.66
27.97
143.14
Standard deviation
0.91
1.55
7.21
0.93
1.5
4.65
C V (%)
6
5.03
5.05
6.81
5.36
3.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PSMA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PSMA in samples. No significant cross-reactivity or interference between Human PSMA and analogues was observed.
