The Human PSA (Prostate-Specific Antigen) ELISA Kit is specifically designed for the precise detection of PSA levels in human serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for various research applications.PSA is a protein that is primarily produced by prostate gland cells and is commonly used as a biomarker for prostate cancer screening and monitoring. Elevated levels of PSA can indicate the presence of prostate cancer or other prostate-related conditions, highlighting the importance of reliable PSA testing.
This ELISA kit provides researchers with a valuable tool for studying prostate cancer, monitoring disease progression, and evaluating treatment effectiveness. With its ease of use and robust performance, the Human PSA ELISA Kit from Assay Genie is an essential resource for advancing research in the field of prostate cancer and improving patient outcomes.
Product Name:
Human PSA (Prostate Specific Antigen) ELISA Kit
SKU:
HUES01377
Target:
Human PSA (Prostate Specific Antigen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PSA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PSA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PSA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PSA. You can calculate the concentration of Human PSA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
85-96
87-97
Average (%)
101
91
92
1:4
Range (%)
89-102
85-99
86-100
Average (%)
94
90
91
1:8
Range (%)
91-106
84-95
85-97
Average (%)
98
89
91
1:16
Range (%)
88-99
83-98
87-99
Average (%)
93
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.88
7.1
1.05
2.78
7.63
Standard deviation
0.07
0.17
0.26
0.05
0.13
0.34
C V (%)
7
5.9
3.66
4.76
4.68
4.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PSA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PSA in samples. No significant cross-reactivity or interference between Human PSA and analogues was observed.
