The Human Protocadherin-15 (PCDH15) ELISA Kit is a reliable and sensitive assay for the precise measurement of PCDH15 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high specificity and reproducibility, ensuring accurate results that are suitable for a wide range of research applications.PCDH15 is a critical protein involved in cell-cell adhesion and communication, particularly in the development and function of the auditory and vestibular systems.
Mutations in the PCDH15 gene are associated with various forms of deafness and balance disorders, making it a key biomarker for the study of these conditions and the development of potential therapies.Overall, the Human Protocadherin-15 (PCDH15) ELISA Kit is a valuable tool for researchers seeking to investigate the role of PCDH15 in auditory and vestibular function, as well as its potential implications in the treatment of hearing and balance disorders.
Product Name:
Human Protocadherin 15 (PCDH15) ELISA Kit
Product Code:
HUDL02199
Size:
96 Assays
Target:
PCDH15
Synonyms:
USH1F, DFNB23, CDHR15, Deafness,Autosomal Recessive 23, Cadherin-Related Family Member 15
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
