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Human Protein Zero, Myelin (MPZ) ELISA Kit

SKU:
HUDL01901
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P25189
Sensitivity:
0.052ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
P0,DSS,CHM,CMT4E,CMT1,CMT1B,CMT2I,CMT2J,CMTDI3,HMSNIB,MPP,Myelin Protein Zero,Charcot-Marie-Tooth Neuropathy 1B,Myelin peripheral protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Protein Zero, Myelin (MPZ) ELISA Kit

The Human Protein Zero Myelin (MPZ) ELISA Kit is specifically designed for the precise measurement of MPZ levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.MPZ, also known as Protein Zero, is a key component of myelin protein in the nervous system, playing a critical role in the structure and function of myelin sheaths. Abnormalities in MPZ levels are associated with neurological disorders such as Charcot-Marie-Tooth disease, making it a valuable biomarker for studying these conditions and developing potential treatments.

Whether investigating the pathogenesis of neurological diseases or exploring the role of MPZ in myelin formation, the Human Protein Zero Myelin (MPZ) ELISA Kit provides a reliable solution for researchers seeking to advance their understanding of these complex conditions.

Product Name:Human Protein Zero, Myelin (MPZ) ELISA Kit
Product Code:HUDL01901
Size:96 Assays
Target:MPZ
Synonyms:P0, DSS, CHM, CMT4E, CMT1, CMT1B, CMT2I, CMT2J, CMTDI3, HMSNIB, MPP, Myelin Protein Zero, Charcot-Marie-Tooth Neuropathy 1B, Myelin peripheral protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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