The Human Protein Z (PROZ) ELISA Kit is a cutting-edge tool for precise measurement of protein Z levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results that are vital for various research purposes.Protein Z is a key player in the coagulation cascade, contributing to proper blood clotting and thrombosis prevention. Dysregulation of protein Z levels has been linked to various health conditions, including thrombosis, cardiovascular diseases, and pregnancy complications.
By using this ELISA kit, researchers can gain valuable insights into the role of protein Z in these disorders and explore potential therapeutic interventions.In conclusion, the Human Protein Z (PROZ) ELISA Kit is a valuable asset for researchers studying coagulation disorders and seeking to understand the complex mechanisms underlying thrombosis-related diseases. With its high-performance features and versatility, this kit is a must-have tool for advancing scientific knowledge in the field of hemostasis and thrombosis.
Product Name:
Human Protein Z (PROZ) ELISA Kit
Product Code:
HUDL02413
Size:
96 Assays
Target:
PROZ
Synonyms:
PRO-Z, PZ, Vitamin K Dependent Protein Z, VKDPZ, VKDP-Z
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
