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Human Protein Tyrosine Phosphatase Receptor Type S (PTPRS) ELISA Kit

SKU:
HUDL02463
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13332
Sensitivity:
0.27ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
PTPR-S,PTPSIGMA,PTP SIGMA,Receptor-type tyrosine-protein phosphatase sigma
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human Protein Tyrosine Phosphatase Receptor Type S (PTPRS) ELISA Kit

The Human Protein Tyrosine Phosphatase Receptor Type S (PTPRS) ELISA Kit is specially designed to accurately measure the levels of PTPRS in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and consistent results, making it perfect for a wide range of research applications.PTPRS is a vital protein involved in cell signaling and regulation, playing a crucial role in processes such as cell growth, differentiation, and immune response.

Dysregulation of PTPRS has been implicated in various diseases, including cancer, autoimmune disorders, and neurological conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Get reliable data and insights into PTPRS levels with the Human Protein Tyrosine Phosphatase Receptor Type S ELISA Kit, an essential tool for researchers in the field of molecular biology and biochemistry.

Product Name:Human Protein Tyrosine Phosphatase Receptor Type S (PTPRS) ELISA Kit
Product Code:HUDL02463
Size:96 Assays
Target:PTPRS
Synonyms:PTPR-S, PTPSIGMA, PTP SIGMA, Receptor-type tyrosine-protein phosphatase sigma
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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