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Human Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) ELISA Kit

SKU:
HUDL02458
Product Type:
ELISA Kit
Sensitivity:
0.29ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
BPTP3,CFC,NS1,PTP-1D,PTP2C,SH-PTP2,SH-PTP3,SHP2,Noonan Syndrome 1,Protein-tyrosine phosphatase 1D,Protein-tyrosine phosphatase 2C
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

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Human Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) ELISA Kit

The Human Protein Tyrosine Phosphatase Non-Receptor Type 11 (PTPN11) ELISA Kit is specifically designed for the accurate detection of PTPN11 levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PTPN11, also known as SHP2, is an important protein involved in signal transduction pathways and cell growth regulation. Dysregulation of PTPN11 has been linked to various diseases including cancer, developmental disorders, and metabolic conditions, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.

With easy-to-follow protocols and a user-friendly format, the Human PTPN11 ELISA Kit is an essential tool for researchers looking to accurately quantify PTPN11 levels in biological samples and further understand its role in disease pathogenesis.

Product Name:Human Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) ELISA Kit
Product Code:HUDL02458
Size:96 Assays
Target:PTPN11
Synonyms:BPTP3, CFC, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, Noonan Syndrome 1, Protein-tyrosine phosphatase 1D, Protein-tyrosine phosphatase 2C
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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