null

Human Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) ELISA Kit

SKU:
HUDL02402
Product Type:
ELISA Kit
Sensitivity:
0.125ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HYRC,HYRC1,DNPK1,p350,DNAPK,XRCC7,DNA-PKcs
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) ELISA Kit

The Human Protein Kinase DNA-activated Catalytic Polypeptide (PRKDC) ELISA Kit is a powerful tool for researchers looking to accurately measure PRKDC levels in human samples. PRKDC is a key player in DNA repair and maintenance, and dysregulation of this protein has been implicated in various diseases, including cancer and autoimmune disorders.This ELISA kit boasts high sensitivity and specificity, enabling researchers to obtain reliable and reproducible results with minimal sample input.

It is compatible with serum, plasma, and cell culture supernatants, making it versatile for a wide range of experimental setups.By utilizing the Human PRKDC ELISA Kit, researchers can gain valuable insights into the role of PRKDC in DNA damage response pathways and disease pathogenesis. This kit is an indispensable tool for understanding the intricate mechanisms underlying diseases and developing targeted therapies.

Product Name:Human Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) ELISA Kit
Product Code:HUDL02402
Size:96 Assays
Target:PRKDC
Synonyms:HYRC, HYRC1, DNPK1, p350, DNAPK, XRCC7, DNA-PKcs
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products