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Human Protein Kinase B Beta (PKBb) ELISA Kit

SKU:
HUDL02301
Product Type:
ELISA Kit
Sensitivity:
0.132ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
AKT2,PKBBETA,PRKB-B,RAC-BETA,RAC-Beta Serine/Threonine-Protein Kinase,V-Akt Murine Thymoma Viral Oncogene Homolog 2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Protein Kinase B Beta (PKBb) ELISA Kit

The Human Protein Kinase B Beta (PKBβ) ELISA Kit is specifically designed for the precise measurement of PKBβ levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, leading to accurate and consistent results, making it a valuable tool for various research purposes.Protein Kinase B Beta, also known as Akt2, is a key enzyme involved in multiple cellular processes, including cell growth, proliferation, and survival.

Dysregulation of PKBβ has been linked to various diseases, such as cancer and diabetes, highlighting its importance as a therapeutic target and biomarker in these conditions.Whether studying signaling pathways, drug development, or disease mechanisms, the Human Protein Kinase B Beta (PKBβ) ELISA Kit provides researchers with a reliable method to quantify and analyze PKBβ levels, ultimately advancing our understanding of its role in health and disease.

Product Name:Human Protein Kinase B Beta (PKBb) ELISA Kit
Product Code:HUDL02301
Size:96 Assays
Target:PKBb
Synonyms:AKT2, PKBBETA, PRKB-B, RAC-BETA, RAC-Beta Serine/Threonine-Protein Kinase, V-Akt Murine Thymoma Viral Oncogene Homolog 2
Detection Method:Competition
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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