Human Proteasome 26S Subunit, Non ATPase 10 (PSMD10) ELISA Kit
The Human Proteasome 26S Subunit Non-ATPase 10 (PSMD10) ELISA Kit is specifically designed for the precise measurement of PSMD10 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.PSMD10 is a critical component of the 26S proteasome complex, essential for protein degradation in the cell. Dysregulation of PSMD10 has been implicated in various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
Therefore, the accurate measurement of PSMD10 levels is crucial for understanding its role in disease pathogenesis and developing potential therapeutic interventions.Overall, the Human PSMD10 ELISA Kit provides a valuable tool for researchers studying protein degradation pathways and investigating the molecular mechanisms underlying various diseases. Its reliability and precision make it an indispensable asset for advancing scientific knowledge and exploring new avenues for potential treatments.
Product Name:
Human Proteasome 26S Subunit, Non ATPase 10 (PSMD10) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
