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Human Protease, Serine 2 (PRSS2) ELISA Kit

SKU:
HUDL02418
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07478
Sensitivity:
0.21ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
TRY2,Trypsin 2,Serine Endopeptidases,Anionic trypsinogen
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

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Human Protease, Serine 2 (PRSS2) ELISA Kit

The Human Protease Serine 2 (PRSS2) ELISA Kit is a powerful tool for accurately measuring levels of PRSS2 in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for various research applications.PRSS2 is an important serine protease involved in a variety of physiological processes, including digestion, blood clotting, and immunity.

Dysregulation of PRSS2 has been linked to various diseases, such as cancer, inflammatory disorders, and infections, making it a valuable biomarker for understanding these conditions and developing potential therapeutic interventions.Overall, the Human Protease Serine 2 (PRSS2) ELISA Kit offers researchers a precise and efficient method for studying PRSS2 levels in human samples, advancing our understanding of its role in health and disease.

Product Name:Human Protease, Serine 2 (PRSS2) ELISA Kit
Product Code:HUDL02418
Size:96 Assays
Target:PRSS2
Synonyms:TRY2, Trypsin 2, Serine Endopeptidases, Anionic trypsinogen
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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