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Human Protease, Serine 1 (PRSS1) ELISA Kit

SKU:
HUDL02416
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07477
Sensitivity:
3.9U/L
Range:
7.812-500U/L
ELISA Type:
Sandwich
Synonyms:
TRP1,TRY1,TRY4,TRYP1,Trypsin 1,Cationic Trypsinogen,Beta-trypsin,Serine protease 1,Trypsin I
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Protease, Serine 1 (PRSS1) ELISA Kit

The Human PRSS1 (Protease Serine 1) ELISA Kit is a reliable and accurate tool for the detection of PRSS1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.PRSS1 is a serine protease that plays a crucial role in the regulation of digestion by breaking down proteins in the pancreas.

Mutations in the PRSS1 gene have been linked to hereditary pancreatitis, making it a key biomarker for studying pancreatic disorders and developing potential treatments.With its high-quality components and user-friendly design, the Human PRSS1 ELISA Kit is an essential tool for researchers studying pancreatic diseases and seeking to better understand the role of PRSS1 in human health.

Product Name:Human Protease, Serine 1 (PRSS1) ELISA Kit
Product Code:HUDL02416
Size:96 Assays
Target:PRSS1
Synonyms:TRP1, TRY1, TRYP1, Trypsin 1, Cationic Trypsinogen, Beta-trypsin, Serine protease 1, Trypsin I
Detection Method:Sandwich
Range:7.812-500U/L
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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