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Human Protease Activated Receptor 1 (PAR1)ELISA Kit

SKU:
HUDL02186
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P25116
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
FIIR,F2r,CF2R,HTR,PAR1,TR,Coagulation Factor II Receptor,Thrombin Receptor
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Protease Activated Receptor 1 (PAR1) ELISA Kit

The Human Protease-Activated Receptor 1 (PAR1) ELISA Kit is a powerful tool for the accurate measurement of PAR1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.PAR1 is a key receptor involved in mediating cellular responses to various stimuli, including thrombin activation and inflammation. Dysregulation of PAR1 signaling has been implicated in various diseases, including cardiovascular disorders, cancer, and inflammatory conditions, making it a crucial target for therapeutic intervention.

By accurately quantifying PAR1 levels in biological samples, researchers can gain valuable insights into the role of this receptor in disease pathology and identify potential therapeutic strategies. The Human PAR1 ELISA Kit provides a convenient and efficient method for studying PAR1 biology and advancing our understanding of its therapeutic potential.

Product Name:Human Protease Activated Receptor 1 (PAR1)ELISA Kit
Product Code:HUDL02186
Size:96 Assays
Target:PAR1
Synonyms:FIIR, F2r, CF2R, HTR, PAR1, TR, Coagulation Factor II Receptor, Thrombin Receptor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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