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Human Prostaglandin I Synthase (PTGIS) ELISA Kit

SKU:
HUDL02446
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q16647
Sensitivity:
0.64ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
PGIS,CYP8A1,Prostacyclin Synthase,Prostacycline Synthetase,Prostagladin I2 Synthetase,PGI2 Synthetase,Cytochrome P450,Family 8,Subfamily A,Polypeptide 1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Prostaglandin I Synthase (PTGIS) ELISA Kit

The Human Prostaglandin I Synthase ELISA Kit is specifically designed for the precise measurement of prostaglandin I synthase levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.Prostaglandin I synthase is an enzyme that plays a crucial role in the production of prostacyclin, a potent vasodilator and inhibitor of platelet aggregation.

Dysregulation of prostaglandin I synthase has been linked to numerous inflammatory and cardiovascular conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.With its advanced technology and superior performance, the Human Prostaglandin I Synthase ELISA Kit is an essential tool for researchers looking to explore the role of prostaglandin I synthase in health and disease.

Product Name:Human Prostaglandin I Synthase (PTGIS) ELISA Kit
Product Code:HUDL02446
Size:96 Assays
Target:PTGIS
Synonyms:PGIS, CYP8A1, Prostacyclin Synthase, Prostacycline Synthetase, Prostagladin I2 Synthetase, PGI2 Synthetase, Cytochrome P450,Family 8,Subfamily A,Polypeptide 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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