Human Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit
The Human Prostaglandin Endoperoxide Synthase-2 (PTGS2) ELISA Kit is a cutting-edge tool designed for the precise measurement of PTGS2 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides accurate and consistent results, making it an invaluable resource for various research purposes.PTGS2, also known as cyclooxygenase-2 (COX-2), is an important enzyme involved in the synthesis of prostaglandins, which play a critical role in inflammation, pain, and other physiological processes. Dysregulation of PTGS2 has been implicated in numerous diseases, including cancer, arthritis, and cardiovascular disorders, highlighting its significance as a potential therapeutic target and biomarker.
By utilizing the Human PTGS2 ELISA Kit, researchers can gain valuable insights into the role of PTGS2 in health and disease, paving the way for the development of novel treatment strategies and diagnostic tools. With its advanced technology and reliable performance, this kit offers a comprehensive solution for investigating the complex mechanisms underlying PTGS2 function and its implication in various pathological conditions.
Product Name:
Human Prostaglandin Endoperoxide Synthase 2 (PTGS2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
